Abstract: The environmental isotopes in the major aquifer of Liuqiao coal mining area have been tested and analyzed.The values of δ¹⁸O of the aquifer are between -5.51‰~-10.87‰,average value is -9.34‰,and the values of δD of the aquifer are between -56.3 ‰~-83.4‰,average value is -71.48‰.The values of the δD and δ¹⁸O decrease with the decrease of depth.The general characteristics of the surface water,the first aquifer and second aquifer of the Quarternary,the coalaquifer,the Taiyuan Formation limestone aquifer and the Ordovician limestone aquifer in the mining district are analyzed.The supply and runoff condition and the relation between the Ordovician limestone aquifer and other aquifers in the area are studied.