Abstract: Hydrodynamic dispersion coefficient is an important parameter in the study of solute transport in groundwater. In order to understand the transport law of pollutants in groundwater, the particle swarm optimization(PSO) algorithm based on bionic principle is used to solve the hydrodynamic dispersion coefficient of phreatic aquifer under natural flow field in the dispersion test site of Jiang'an Campus of Sichuan University. Compared with the least-square method and the standard curve comparison method, the results show that the results of the standard curve method are subject to subjective influence, and the errors are relatively large. The results of least square method fit well with the measured data, but the calculation process is relatively complex. Particle swarm optimization(PSO) is a reliable solution method, has the highest accuracy, faster calculation, good convergence and stability.