Abstract: The land barren, grassland degradation and difficult ecological restoration are serious in the open-pit mining area of eastern China. The symbiosis between arbuscular mycorrhizal fungi(AMF) and Hippophae rhamnoides and the effect of mycorrhiza on the development of Hippophae rhamnoides root system and the improvement of soil in the dumping site were analyzed. It was found that the plant height and crown width of inoculated AMF(+M) were increased by 20% and 21%, respectively. The root length and root volume of +M were 1.46 and 1.97 times of CK, respectively. The content of Glomalin in the rhizosphere soil of Hippophae rhamnoides in +M was 3.22 mg/g, which was significantly higher than 2.16 mg/g in CK. The phosphatase activity of +M was 1.76 times that of CK, and the content of available phosphorus was similar. The results show that it is feasible to use microorganism to promote soil improvement and ecological restoration of the open-pit mine dump.